Key Informatics Challenges in Metabolomics

Key Informatics Challenges in Metabolomics

n  Spectra -> Lists

n  Data integrity and quality

n  Data alignment and normalization

n  Data reduction and classification

n  Assessment of significance

n  Metabolite identification/quantification

n  Lists -> Pathways & Biomarkers

n  Pathway mapping and identification

n  Biological interpretation

Metabolomics can show amplified biochemical status

•          Metabolome : End products of gene expression and can define the biochemical phenotype of a cell or tissue.

•           Quantitative and qualitative measurements of large numbers of cellular metabolites  thus provide a broad view of the biochemical status of an organism that can be used to monitor and assess gene function

Age and Variety Effect on Brassica Metabolome

•       Whaterver you want to see a factor, plant can still grow in your experimental period

•       The change can happen in a week, also in an hour

•       Be careful to choose the same variety