HYBRID SEED PRODUCTION
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HYBRID SEED PRODUCTION IN HOT PEPPER
´ Self pollination
´ Cross pollination up to 30-40%
´ Up to 80%
´ Population of Pollinator
´ Honeybee, bumblebee, Housefly, Ant, Wasp,
Thrips
´ 2-3 bee hives for 1 acre seed production plot
´ Adjoining crops
´ Sunflower, onion, cucurbits etc.
POLLINATION CONTROL
MECHANISMS
´ Male
Sterility System
´ Genetic
male sterility
´ Cytoplasmic
genetic male sterility
HAND POLLINATION
´ Parents
´ Male parent
´ Female parent
´ Manual emasculation and crossing
´ Tedious job
´ Not economical
´ Expert labor
USE OF MALE STERILITY
´ Three lines system / ABR line system
´ Genetic male sterility
´ Cytoplasmic genetic male sterility
´ A line, Male sterile line, Female line
´ R line, Fertility restorer line, Male line
´ B line, Isoline of A line, Maintainer line
SELECTION OF FIELD
´ No chilli or bell pepper crop during last
year
´ Sandy loam soil
´ Well drained
´ Low lying, water logged fields should be
avoided
LAND PREPARATION
´ 4-5 ploughings and plankings
´ Ridges 60 cm apart
SEED RATE
´ True to type, good germination, vigor and
health
´ Bold and uniform color
´ Damaged seed (Black, shrivelled, light in
weight, black spot in centre
´ Female parent (300 g) , Male parent (200 g) /
Acre
NURSERY RAISING
•
12 msq
land for nursery of 1 acre
•
1 m wide
and 20 cm raised beds
•
Seed
sown 5 cm deep in soil
•
5-8 cm
apart rows
FERTILIZATION AND IRRIGATION
´ N : P : K requirement : 66 : 46 : 25 Kg/Acre
´ 2 Bags DAP and 1 Bag Potash during land
preparation
´ ½ Bag Urea at seedling establishment
´ Remaining nitrogen with one moth intervals
´ Irrigation weekly or as required
´ 3-4 Hoeing for weeds eradication and soil
aeration
´ Before flowering, earthing up of plants
MAINTENANCE OF A and B LINE
´ Male sterile maintainer block
´ Male sterile plant (msms) cross with heterozygous male fertile
plant (Msms)
´ Male sterile and male fertile plants in 1:1
ratio
´ On ridges, 60-70 cm apart at 4o cm seedling
distance
HYBRID SEED PRODUCTION
´ Isolated field
´ 400 m isolation
´ Alternate plantation of female (A) line and
male (R) line
´ 2 rows of female lines to one row of male
line (2 : 1)
´ Row to row distance 60 cm
´ Plant to plant distance
´ Female line
20 cm
´ Male line 40 cm
PREPARATION OF FEMALE LINE FOR CROSS POLLINATION
´ 50% male fertile plants in female line
´ Rouging of male fertile plants from female
(A) line
´ Before pollen shedding
´ From morning to evening, up to 10 days
´ Tagging of male sterile line
´ Remove all fruit set from male and female
parents three to four days after this operation
IDENTIFICATION OF MALE STERILA AND MALE FERTILE PLANTS IN
FEMALE (A) LINE
´ Blooming stage
´ Anthesis between 5-7 am
´ Dehiscence
just after Anthesis
´ After dehiscence, check presence or absence
of pollen in flower
´ Differentiating characters
Pollen, anther
color, anther size, anther bursting, fruit set
HARVESTING AND SEED EXTRACTION
´ Red ripe fruit
´ Through drying in the sun
´ Crushing and winnowing
´ Manual extraction by hands
´ Axial flow vegetable seed extraction machine
´ Seed drying and storage (6-8% moisture
contents)
Crop
|
Plants required to produce 1 Kg of seed
|
Average yield of seed /Acre (Kg)
|
Hot Pepper
|
64-256
|
25-100
|
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